Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata.
Identifieur interne : 000F54 ( Main/Exploration ); précédent : 000F53; suivant : 000F55Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata.
Auteurs : Samira R. Aili [Australie] ; Axel Touchard [France] ; Frédéric Petitclerc [France] ; Alain Dejean [France] ; Jérôme Orivel [France] ; Matthew P. Padula [Australie] ; Pierre Escoubas [France] ; Graham M. Nicholson [Australie]Source :
- Journal of proteome research [ 1535-3907 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Peptides, Protéines d'insecte, Venins de fourmi.
- pathogénicité : Fourmis.
- Animaux, Fourmis, Protéomique, Spectrométrie de masse en tandem, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Ant Venoms, Insect Proteins, Peptides.
- chemistry : Ants.
- methods : Proteomics.
- pathogenicity : Ants.
- Animals, Electrophoresis, Gel, Two-Dimensional, Tandem Mass Spectrometry.
Abstract
Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.
DOI: 10.1021/acs.jproteome.6b00948
PubMed: 28118015
Affiliations:
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Aili</name><affiliation wicri:level="1"><nlm:affiliation>Neurotoxin Research Group, School of Life Sciences, University of Technology Sydney, New South Wales 2007, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Neurotoxin Research Group, School of Life Sciences, University of Technology Sydney, New South Wales 2007</wicri:regionArea><wicri:noRegion>New South Wales 2007</wicri:noRegion></affiliation></author><author><name sortKey="Touchard, Axel" sort="Touchard, Axel" uniqKey="Touchard A" first="Axel" last="Touchard">Axel Touchard</name><affiliation wicri:level="1"><nlm:affiliation>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou</wicri:regionArea><wicri:noRegion>97310 Kourou</wicri:noRegion><wicri:noRegion>97310 Kourou</wicri:noRegion></affiliation></author><author><name sortKey="Petitclerc, Frederic" sort="Petitclerc, Frederic" uniqKey="Petitclerc F" first="Frédéric" last="Petitclerc">Frédéric Petitclerc</name><affiliation wicri:level="1"><nlm:affiliation>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou</wicri:regionArea><wicri:noRegion>97310 Kourou</wicri:noRegion><wicri:noRegion>97310 Kourou</wicri:noRegion></affiliation></author><author><name sortKey="Dejean, Alain" sort="Dejean, Alain" uniqKey="Dejean A" first="Alain" last="Dejean">Alain Dejean</name><affiliation wicri:level="1"><nlm:affiliation>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou</wicri:regionArea><wicri:noRegion>97310 Kourou</wicri:noRegion><wicri:noRegion>97310 Kourou</wicri:noRegion></affiliation></author><author><name sortKey="Orivel, Jerome" sort="Orivel, Jerome" uniqKey="Orivel J" first="Jérôme" last="Orivel">Jérôme Orivel</name><affiliation wicri:level="1"><nlm:affiliation>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS, UMR Ecologie des Forêts de Guyane (EcoFoG), AgroParisTech, Cirad, INRA, Université des Antilles, Université de Guyane, Université des Antilles , 97310 Kourou</wicri:regionArea><wicri:noRegion>97310 Kourou</wicri:noRegion><wicri:noRegion>97310 Kourou</wicri:noRegion></affiliation></author><author><name sortKey="Padula, Matthew P" sort="Padula, Matthew P" uniqKey="Padula M" first="Matthew P" last="Padula">Matthew P. Padula</name><affiliation wicri:level="1"><nlm:affiliation>Proteomics Core Facility, Faculty of Science, University of Technology Sydney, New South Wales 2007, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Proteomics Core Facility, Faculty of Science, University of Technology Sydney, New South Wales 2007</wicri:regionArea><wicri:noRegion>New South Wales 2007</wicri:noRegion></affiliation></author><author><name sortKey="Escoubas, Pierre" sort="Escoubas, Pierre" uniqKey="Escoubas P" first="Pierre" last="Escoubas">Pierre Escoubas</name><affiliation wicri:level="1"><nlm:affiliation>VenomeTech , 473 Route des Dolines - Villa 3, Valbonne 06560, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>VenomeTech , 473 Route des Dolines - Villa 3, Valbonne 06560</wicri:regionArea><wicri:noRegion>06560</wicri:noRegion><wicri:noRegion>06560</wicri:noRegion></affiliation></author><author><name sortKey="Nicholson, Graham M" sort="Nicholson, Graham M" uniqKey="Nicholson G" first="Graham M" last="Nicholson">Graham M. Nicholson</name><affiliation wicri:level="1"><nlm:affiliation>Neurotoxin Research Group, School of Life Sciences, University of Technology Sydney, New South Wales 2007, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Neurotoxin Research Group, School of Life Sciences, University of Technology Sydney, New South Wales 2007</wicri:regionArea><wicri:noRegion>New South Wales 2007</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of proteome research</title><idno type="eISSN">1535-3907</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Ant Venoms (analysis)</term><term>Ants (chemistry)</term><term>Ants (pathogenicity)</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Insect Proteins (analysis)</term><term>Peptides (analysis)</term><term>Proteomics (methods)</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Fourmis ()</term><term>Fourmis (pathogénicité)</term><term>Peptides (analyse)</term><term>Protéines d'insecte (analyse)</term><term>Protéomique ()</term><term>Spectrométrie de masse en tandem</term><term>Venins de fourmi (analyse)</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Ant Venoms</term><term>Insect Proteins</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptides</term><term>Protéines d'insecte</term><term>Venins de fourmi</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Ants</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Ants</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Fourmis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electrophoresis, Gel, Two-Dimensional</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Fourmis</term><term>Protéomique</term><term>Spectrométrie de masse en tandem</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Aili, Samira R" sort="Aili, Samira R" uniqKey="Aili S" first="Samira R" last="Aili">Samira R. Aili</name></noRegion><name sortKey="Nicholson, Graham M" sort="Nicholson, Graham M" uniqKey="Nicholson G" first="Graham M" last="Nicholson">Graham M. Nicholson</name><name sortKey="Padula, Matthew P" sort="Padula, Matthew P" uniqKey="Padula M" first="Matthew P" last="Padula">Matthew P. Padula</name></country><country name="France"><noRegion><name sortKey="Touchard, Axel" sort="Touchard, Axel" uniqKey="Touchard A" first="Axel" last="Touchard">Axel Touchard</name></noRegion><name sortKey="Dejean, Alain" sort="Dejean, Alain" uniqKey="Dejean A" first="Alain" last="Dejean">Alain Dejean</name><name sortKey="Escoubas, Pierre" sort="Escoubas, Pierre" uniqKey="Escoubas P" first="Pierre" last="Escoubas">Pierre Escoubas</name><name sortKey="Orivel, Jerome" sort="Orivel, Jerome" uniqKey="Orivel J" first="Jérôme" last="Orivel">Jérôme Orivel</name><name sortKey="Petitclerc, Frederic" sort="Petitclerc, Frederic" uniqKey="Petitclerc F" first="Frédéric" last="Petitclerc">Frédéric Petitclerc</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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